Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-34690 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRMT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T.
- Concentration
- 0.69 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRMT2 using 30 µg of 293T lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PRMT2 polyclonal antibody (Product # PA5-34690) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PRMT2 Polyclonal Antibody (Product # PA5-34690). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRMT2 Polyclonal Antibody (Product # PA5-34690) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRMT2 Polyclonal Antibody detects PRMT2 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT2 protein stained by PRMT2 Polyclonal Antibody (Product # PA5-34690) diluted at 1:500. Blue: Hoechst 33342 staining.
