- MA1-80446 - Invitrogen Antibodies S100A9 antibody

Submitted references High Numbers of CD163-Positive Macrophages in the Fibrotic Region of Exuberant Granulation Tissue in Horses.

Du Cheyne C, Martens A, De Spiegelaere W
Animals : an open access journal from MDPI 2021 Sep 18;11(9)

Equine cervical remodeling during placentitis and the prepartum period: a transcriptomic approach.

El-Sheikh Ali H, Scoggin KE, Ruby R, Loynachan A, Boakari Y, Fernandes C, Dini P, Fedorka CE, Loux SC, Esteller-Vico A, Ball BA
Reproduction (Cambridge, England) 2021 May 5;161(6):603-621

Reinforcement of Colonic Anastomosis with Improved Ultrafine Nanofibrous Patch: Experiment on Pig.

Rosendorf J, Klicova M, Cervenkova L, Horakova J, Klapstova A, Hosek P, Palek R, Sevcik J, Polak R, Treska V, Chvojka J, Liska V
Biomedicines 2021 Jan 21;9(2)

Experimental fortification of intestinal anastomoses with nanofibrous materials in a large animal model.

Rosendorf J, Horakova J, Klicova M, Palek R, Cervenkova L, Kural T, Hosek P, Kriz T, Tegl V, Moulisova V, Tonar Z, Treska V, Lukas D, Liska V
Scientific reports 2020 Jan 24;10(1):1134

Neutrophils express pro- and anti-inflammatory cytokines in granulomas from Mycobacterium tuberculosis-infected cynomolgus macaques.

Gideon HP, Phuah J, Junecko BA, Mattila JT
Mucosal immunology 2019 Nov;12(6):1370-1381

A Skin Rejection Grading System for Vascularized Composite Allotransplantation in a Preclinical Large Animal Model.

Etra JW, Grzelak MJ, Fidder SAJ, Kolegraff K, Bonawitz S, Shores J, Oh B, Cooney DS, Beck SE, Brandacher G
Transplantation 2019 Jul;103(7):1385-1391

Circulating monocytes accelerate acute liver failure by IL-6 secretion in monkey.

Guo G, Zhu Y, Wu Z, Ji H, Lu X, Zhou Y, Li Y, Cao X, Lu Y, Talbot P, Liao J, Shi Y, Bu H
Journal of cellular and molecular medicine 2018 Sep;22(9):4056-4067

Differential Interstrain Susceptibility to Vertebrobasilar Dolichoectasia in a Mouse Model.

Zhu YQ, Xing H, Dai D, Kallmes DF, Kadirvel R
AJNR. American journal of neuroradiology 2017 Mar;38(3):611-616

Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques.

Phuah J, Wong EA, Gideon HP, Maiello P, Coleman MT, Hendricks MR, Ruden R, Cirrincione LR, Chan J, Lin PL, Flynn JL
Infection and immunity 2016 May;84(5):1301-1311

Polymeric embolization coil of bilayered polyvinyl alcohol strand for therapeutic vascular occlusion: a feasibility study in canine experimental vascular models.

Jung SC, Choi SH, Cho HR, Lee TH, Kim TY, Jeong W, Rhee K, Jho JY, Kim JH, Han MH
Journal of vascular and interventional radiology : JVIR 2015 Jan;26(1):117-23

Morphological and physiological retinal degeneration induced by intravenous delivery of vitamin A dimers in rabbits.

Penn J, Mihai DM, Washington I
Disease models & mechanisms 2015 Feb;8(2):131-8

Identification of the critical therapeutic entity in secreted Hsp90α that promotes wound healing in newly re-standardized healthy and diabetic pig models.

O'Brien K, Bhatia A, Tsen F, Chen M, Wong AK, Woodley DT, Li W
PloS one 2014;9(12):e113956

Short-term safety and efficacy of the biodegradable iron stent in mini-swine coronary arteries.

Wu C, Qiu H, Hu XY, Ruan YM, Tian Y, Chu Y, Xu XL, Xu L, Tang Y, Gao RL
Chinese medical journal 2013;126(24):4752-7

Xenotransplantation of human unrestricted somatic stem cells in a pig model of acute myocardial infarction.

Gahremanpour A, Vela D, Zheng Y, Silva GV, Fodor W, Cardoso CO, Baimbridge F, Fernandes MR, Buja LM, Perin EC
Xenotransplantation 2013 Mar-Apr;20(2):110-22

Microenvironments in tuberculous granulomas are delineated by distinct populations of macrophage subsets and expression of nitric oxide synthase and arginase isoforms.

Mattila JT, Ojo OO, Kepka-Lenhart D, Marino S, Kim JH, Eum SY, Via LE, Barry CE 3rd, Klein E, Kirschner DE, Morris SM Jr, Lin PL, Flynn JL
Journal of immunology (Baltimore, Md. : 1950) 2013 Jul 15;191(2):773-84

Transplantation with autologous mesenchymal stem cells after acute myocardial infarction evaluated by magnetic resonance imaging: an experimental study.

Lu M, Zhao S, Liu Q, Jiang S, Song P, Qian H, Zhang Y, Ling J, Yan C, Cheng H, Ma N, Zhao H, Liu Y
Journal of thoracic imaging 2012 Mar;27(2):125-35

Gene expression profile of vascular endothelial growth factor (VEGF) and its receptors in various cell types of the canine lymph node using laser capture microdissection (LCM).

Jais A, Klein D, Wolfesberger B, Walter I
Veterinary immunology and immunopathology 2011 Apr 15;140(3-4):207-14

Insulin increases resistance to burn wound infection-associated sepsis.

Gauglitz GG, Toliver-Kinsky TE, Williams FN, Song J, Cui W, Herndon DN, Jeschke MG
Critical care medicine 2010 Jan;38(1):202-8

Chronic intake of a high-cholesterol diet resulted in hepatic steatosis, focal nodular hyperplasia and fibrosis in non-obese mice.

Sumiyoshi M, Sakanaka M, Kimura Y
The British journal of nutrition 2010 Feb;103(3):378-85

The performance of photooxidatively crosslinked acellular bovine jugular vein conduits in the reconstruction of connections between pulmonary arteries and right ventricles.

Lü WD, Zhang M, Wu ZS, Hu TH, Xu ZJ, Liu W, Hu YR
Biomaterials 2010 Apr;31(10):2934-43

Characterization of a preclinical model of chronic ischemic wound.

Roy S, Biswas S, Khanna S, Gordillo G, Bergdall V, Green J, Marsh CB, Gould LJ, Sen CK
Physiological genomics 2009 May 13;37(3):211-24

Role of cardiopulmonary bypass and arrested heart status in the early cell distribution after intracoronary infusion of bone marrow stromal cells.

Song P, Zhang H, Lu MJ, Li J, Liu XW, Wei YJ, Hu SS
The Journal of surgical research 2009 May 1;153(1):66-70

Injection of bone marrow mesenchymal stem cells in the borderline area of infarcted myocardium: heart status and cell distribution.

Zhang H, Song P, Tang Y, Zhang XL, Zhao SH, Wei YJ, Hu SS
The Journal of thoracic and cardiovascular surgery 2007 Nov;134(5):1234-40

Histopathology of spleen allograft rejection in miniature swine.

Dor FJ, Gollackner B, Kuwaki K, Ko DS, Cooper DK, Houser SL
International journal of experimental pathology 2005 Feb;86(1):57-66

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Calprotectin (S100A9) Monoclonal Antibody (MAC387) (Product # MA1-80155) and a 13 kDa band corresponding to Calprotectin (S100A9) was observed in SK-BR-3 and THP-1 but was absent in Raji and SH-SY5Y which are reported to be negative. Expression of Calprotectin (S100A9) was downregulated upon treatment of THP-1 with PMA/TPA (50ng/mL for 24 hours) [10.1073/pnas.0709958105]. Membrane enriched cell extracts (30 µg lysate) of SK-BR-3 (Lane 1), THP-1 (Lane 2), THP-1 treated with PMA/TPA (50ng/mL for 24 hours) (Lane 3), Raji (Lane 4) and SH-SY5Y (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Calprotectin (S100A9) Monoclonal Antibody (MAC387) (Product # MA1-80446) and a 13 kDa band corresponding to Calprotectin (S100A9) was observed in SK-BR-3 and THP-1 but was absent in Raji and SH-SY5Y which are reported to be negative. Expression of Calprotectin (S100A9) was downregulated upon treatment of THP-1 with PMA/TPA (50ng/mL for 24 hours) [10.1073/pnas.0709958105]. Membrane enriched cell extracts (30 µg lysate) of SK-BR-3 (Lane 1), THP-1 (Lane 2), THP-1 treated with PMA/TPA (50ng/mL for 24 hours) (Lane 3), Raji (Lane 4) and SH-SY5Y (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Calprotectin (S100A9) Monoclonal Antibody (MAC387) (Product # MA1-81381) and a 13 kDa band corresponding to S100A9 was observed in THP-1 but was absent in Raji and SH-SY5Y which are reported to be negative. Expression of S100A9 was downregulated upon treatment of THP-1 with PMA/TPA (50ng/mL for 24 hours) [10.1073/pnas.0709958105]. Membrane enriched cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with PMA/TPA (50ng/mL for 24 hours) (Lane 2), Raji (Lane 3) and SH-SY5Y (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Calprotectin (S100A9) was performed using 70% confluent log phase THP-1 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Calprotectin (S100A9) Monoclonal Antibody (MAC387) (Product # MA1-80155) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of THP-1 showing localization to plasma membrane, nucleus and cytoplasm. Panel e shows Raji cells with no expression of Calprotectin (S100A9). Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Calprotectin (S100A9) was performed using 70% confluent log phase THP-1 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Calprotectin (S100A9) Monoclonal Antibody (MAC387) (Product # MA1-80446) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of THP-1 showing localization to plasma membrane, nucleus and cytoplasm. Panel e shows Raji cells with no expression of Calprotectin (S100A9). Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Calprotectin (S100A9) was performed using 70% confluent log phase THP-1 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Calprotectin (S100A9) Monoclonal Antibody (MAC387) (Product # MA1-81381) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of THP-1 showing localization to plasma membrane, nucleus and cytoplasm. Panel e shows Raji cells with no expression of Calprotectin (S100A9). Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Macrophages/Monocytes Antibody was performed on asian elephant lymph node. To expose target proteins, antigen retrieval was performed by pressure cooker tissues for 10 minutes. Following antigen retrieval, endogenous peroxidases were blocked with 3% hydrogen peroxide for 10 min at room temperature. Tissue slides were washed with deionized water and PBS, and then blocked in goat-serum for 30 min at room temperature. Tissues were probed with a Macrophages/Monocytes Antibody (Product # MA1-80446) diluted 1:200 in goat-serum (upper) or mouse IgG as a negative control (bottom) for 1 hour at 23°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using a biotinylated anti-mouse secondary antibody and avidin-conjugated to horseradish peroxidase, then followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Images were taken at 40X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Immunohistochemistry showing the spatial organization of MAC387+ cells and CD163+ cells in horse EGT. The majority of the MAC387 staining was found in the inflammatory regions (indicated in red) near the wound surface. The CD163 staining was more uniformly distributed. Stained pixels were detected using the positive pixel count algorithm in QuPath and encircled in red. Scale bar inserts = 100 um. Red frame: inflammatory regions; green frame: fibrotic regions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Statistical analysis of quantitative assessment of different parameters: (a ) The mean adhesion score for each group, the control group scored the lowest with no statistical significance; ( b ) The volume fractions of vWF positively stained area for each groups showing the level of vascularisation, the three groups show the same quality of scar in this aspect; ( c ) The volume fractions of collagen fibres for each group, the three groups show the same quality of scar in this aspect; ( d ) The volume fractions of MAC387 positive area for each group, showing the inflammatory cells infiltration, the presence of a stitch in the section proves to be the only statistically significant factor, the three groups show the same quality of scar in this aspect as well.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7 Histological section, PCL group, MAC387 staining: Detail of the marginal zone of the material applied, the empty spaces in the shape of the fibres (stereological grid).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 8 Histological staining of explanted anastomoses: (a ) Green trichrome: Control group; ( b ) Green trichrome: PCL group, the empty space on the site of application of the nanomaterial can be seen in the upper layer, surrounded by normal granulation tissue; (c ) Green trichrome: PLCL group, a much thinner empty area can be seen in the upper layer, also surrounded by normal granulation tissue; ( d ) PSR staining, collagen fibres stained yellow, stereological mesh; ( e ) vWF factor staining, the endothelial cells stained brown, stereological grid; ( f ) MAC 387 staining stereology, positive cells stained blue, stereological grid; ( g ) magnification of vWF staining stereology with a positive cross in the upper right corner.

MA1-80446

Antibody data