MA5-17624

antibody from Invitrogen Antibodies

Targeting: S100A9 60B8AG, CAGB, CFAG, CGLB, LIAG, MAC387, MIF, MRP14, NIF, P14

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17624

Provider

Invitrogen Antibodies

Product name

S100A9 Monoclonal Antibody (CF-557)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17624 targets Calgranulin B in FACS applications and shows reactivity with Human samples.

Antibody clone number

CF-557

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-S100A9 Monoclonal Antibody (Product # MA5-17624) and a 13kDa band corresponding to S100A9 was observed in THP-1 but was absent in Raji and SH-SY5Y which are reported to be negative. Expression of S100A9 was downregulated upon treatment of THP-1 with PMA/TPA [10.1073/pnas.0709958105]. Membrane enriched cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with PMA/TPA (50ng/ml for 24 hours) (Lane 2), Raji (Lane 3) and SH-SY5Y (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).