PA5-85587

antibody from Invitrogen Antibodies

Targeting: S100A9 60B8AG, CAGB, CFAG, CGLB, LIAG, MAC387, MIF, MRP14, NIF, P14

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85587

Provider

Invitrogen Antibodies

Product name

S100A9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of S100A9 in A) MCF-7 whole cell lysate, B) MDA-MB-231 whole cell lysate using S100A9 polyclonal antibody (Product # PA5-85587) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 15% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

S100A9 Polyclonal Antibody detects S100A9 protein by western blot analysis. A. 30 µg MCF-7 whole cell lysate/extract. B. 30 µg MDA-MB-231 whole cell lysate/extract.15 % SDS-PAGE. S100A9 Polyclonal Antibody (Product # PA5-85587) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

S100A9 Polyclonal Antibody detects S100A9 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: S100A9 protein stained by S100A9 Polyclonal Antibody (Product # PA5-85587) diluted at 1:250. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

S100A9 Polyclonal Antibody detects S100A9 protein at secreted on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. S100A9 Polyclonal Antibody (Product # PA5-85587) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

S100A9 Polyclonal Antibody detects S100A9 protein at secreted on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. S100A9 Polyclonal Antibody (Product # PA5-85587) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.