- MA5-12213 - Invitrogen Antibodies S100A9 antibody

Submitted references Molecular Insight into TdfH-Mediated Zinc Piracy from Human Calprotectin by Neisseria gonorrhoeae.

Kammerman MT, Bera A, Wu R, Harrison SA, Maxwell CN, Lundquist K, Noinaj N, Chazin WJ, Cornelissen CN
mBio 2020 May 26;11(3)

Widespread Virus Replication in Alveoli Drives Acute Respiratory Distress Syndrome in Aerosolized H5N1 Influenza Infection of Macaques.

Wonderlich ER, Swan ZD, Bissel SJ, Hartman AL, Carney JP, O'Malley KJ, Obadan AO, Santos J, Walker R, Sturgeon TJ, Frye LJ Jr, Maiello P, Scanga CA, Bowling JD, Bouwer AL, Duangkhae PA, Wiley CA, Flynn JL, Wang J, Cole KS, Perez DR, Reed DS, Barratt-Boyes SM
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 15;198(4):1616-1626

Improving the Preoperative Diagnostic Accuracy of Acute Appendicitis. Can Fecal Calprotectin Be Helpful?

Ambe PC, Orth V, Gödde D, Zirngibl H
PloS one 2016;11(12):e0168769

Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques.

Phuah J, Wong EA, Gideon HP, Maiello P, Coleman MT, Hendricks MR, Ruden R, Cirrincione LR, Chan J, Lin PL, Flynn JL
Infection and immunity 2016 May;84(5):1301-1311

Calprotectin could be a potential biomarker for acute appendicitis.

Ambe PC, Gödde D, Bönicke L, Papadakis M, Störkel S, Zirngibl H
Journal of translational medicine 2016 Apr 27;14(1):107

Short-term safety and efficacy of the biodegradable iron stent in mini-swine coronary arteries.

Wu C, Qiu H, Hu XY, Ruan YM, Tian Y, Chu Y, Xu XL, Xu L, Tang Y, Gao RL
Chinese medical journal 2013;126(24):4752-7

Xenotransplantation of human unrestricted somatic stem cells in a pig model of acute myocardial infarction.

Gahremanpour A, Vela D, Zheng Y, Silva GV, Fodor W, Cardoso CO, Baimbridge F, Fernandes MR, Buja LM, Perin EC
Xenotransplantation 2013 Mar-Apr;20(2):110-22

Transplantation with autologous mesenchymal stem cells after acute myocardial infarction evaluated by magnetic resonance imaging: an experimental study.

Lu M, Zhao S, Liu Q, Jiang S, Song P, Qian H, Zhang Y, Ling J, Yan C, Cheng H, Ma N, Zhao H, Liu Y
Journal of thoracic imaging 2012 Mar;27(2):125-35

Insulin increases resistance to burn wound infection-associated sepsis.

Gauglitz GG, Toliver-Kinsky TE, Williams FN, Song J, Cui W, Herndon DN, Jeschke MG
Critical care medicine 2010 Jan;38(1):202-8

Role of cardiopulmonary bypass and arrested heart status in the early cell distribution after intracoronary infusion of bone marrow stromal cells.

Song P, Zhang H, Lu MJ, Li J, Liu XW, Wei YJ, Hu SS
The Journal of surgical research 2009 May 1;153(1):66-70

Dendritic cell sarcomas/tumours of the breast: report of two cases.

Kapucuoglu N, Percinel S, Ventura T, Lang R, Al-Daraji W, Eusebi V
Virchows Archiv : an international journal of pathology 2009 Mar;454(3):333-9

Primary gastric choriocarcinoma in a dog.

Poutahidis T, Doulberis M, Karamanavi E, Angelopoulou K, Koutinas CK, Papazoglou LG
Journal of comparative pathology 2008 Aug-Oct;139(2-3):146-50

Injection of bone marrow mesenchymal stem cells in the borderline area of infarcted myocardium: heart status and cell distribution.

Zhang H, Song P, Tang Y, Zhang XL, Zhao SH, Wei YJ, Hu SS
The Journal of thoracic and cardiovascular surgery 2007 Nov;134(5):1234-40

Effects of ginseng saponins isolated from Red Ginseng roots on burn wound healing in mice.

Kimura Y, Sumiyoshi M, Kawahira K, Sakanaka M
British journal of pharmacology 2006 Jul;148(6):860-70

Histopathology of spleen allograft rejection in miniature swine.

Dor FJ, Gollackner B, Kuwaki K, Ko DS, Cooper DK, Houser SL
International journal of experimental pathology 2005 Feb;86(1):57-66

Comparison of the morphological changes of restenosis after the implantation of various types of stents in a swine model.

Yorozuya M, Suzuki H, Iso Y, Shibata M, Nakatani M, Koba S, Murakami M, Katagiri T, Takeyama Y
Coronary artery disease 2002 Sep;13(6):305-12

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human tonsil stained with Macrophage antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of macrophages.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 a and b Immunostaining with calprotectin antibody. Note the strong immunohistochemical reaction ( red arrows a ) in the granulocytes and the weak reaction over the apical epithelial membrane ( black arrows , b )

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3 a and b Immunostaining with calprotectin antibody in a specimen with complicated appendicitis with ulceration and intensive immunohistochemical reaction in the lumen ( red arrows ) of appendix due to massive discharge of inflammatory cells

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 a and b Immunostaining with Calprotectin antibody showing an uncomplicated appendicitis with unaltered luminal epithelial architecture. b detail to a . Note the immunohistochemical reaction of neutrophil granulocytes and the absence of immunostaining in the epithelium

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 Control using a specimen from an uninflamed vermiform appendix. Note the absence of immunochemical reaction

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 1 Positive histochemistry with specific anti calprotectin antibody. Positive (gold) immunohistochemical staining (red arrows) with specific anti-calprotectin antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIG 2 hCP and mCP competition dot blot assay. (A) Representative image of direct CP binding assays. N. gonorrhoeae strains FA1090 (WT), MCV661 (TdfH KO), MCV662 (TdfJ KO), and MCV936 (DKO) were grown under Zn-restricted conditions and applied to nitrocellulose membrane at a standardized density. Blots were probed with 0.5 muM hCP or 0.5 muM mCP. CP bound to the surface of cells was detected with an anti-S100A9 monoclonal antibody followed by detection using an anti-mouse IgG conjugated to HRP. (B) Densitometry analysis of results from three independent biological replicates performed in technical triplicate. Densitometry analysis of scanned blots demonstrated a significant reduction in the binding of mCP to affixed gonococci compared to the binding of hCP to the WT strain. (C) Representative image of competitive CP binding assays performed with N. gonorrhoeae strains FA1090 (WT), MCV661 (TdfH KO), MCV662 (TdfJ KO), and MCV936 (DKO). Membranes were probed with either 0.1 muM hCP-HRP alone or a mixture of 0.1 muM hCP-HRP with 0.5 muM or 10 muM hCP or mCP unlabeled competitor. (D) Densitometry analysis of biological triplicate sets of dot blots. Densitometry analysis was accomplished using Bio-Rad's Image Lab software. Significance was calculated using unpaired Student's t tests with comparisons made to FA1090 probed with hCP. Error bars represent SEM (*, P < 0.05; **, P < 0.01).

MA5-12213

Antibody data