Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- NBP2-59292 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal ASH2L Antibody
- Antibody type
- Polyclonal
- Description
- Whole Antiserum.
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASH2L Antibody [NBP2-59292] - A. Western blot was performed on whole cell lysates from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Ash2 , diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the right; the location of the protein of interest (predicted size: 68 kDa) is indicated on the left. B. Western blot was performed on whole cell lysates from mouse neural stem cells (NS), transfected with GFP tagged Ash2, with the antibody against mouse Ash2 , diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the endogenous Ash2 (68 kDa) and of the GFP tagged Ash2 (106 kDa) are indicated on the right.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: ASH2L Antibody [NBP2-59292] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Ash2. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:24,000.