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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 13-0029-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD2 Monoclonal Antibody (RPA-2.10), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.
- Conjugate
- Biotin
- Antibody clone number
- RPA-2.10
- Concentration
- 0.5 mg/mL
Submitted references Type I interferon induces CXCL13 to support ectopic germinal center formation.
Constraints on GPCR Heterodimerization Revealed by the Type-4 Induced-Association BRET Assay.
High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.
T cell-signaling network analysis reveals distinct differences between CD28 and CD2 costimulation responses in various subsets and in the MAPK pathway between resting and activated regulatory T cells.
Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity.
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Cytokine-secreting follicular T cells shape the antibody repertoire.
Denton AE, Innocentin S, Carr EJ, Bradford BM, Lafouresse F, Mabbott NA, Mörbe U, Ludewig B, Groom JR, Good-Jacobson KL, Linterman MA
The Journal of experimental medicine 2019 Mar 4;216(3):621-637
The Journal of experimental medicine 2019 Mar 4;216(3):621-637
Constraints on GPCR Heterodimerization Revealed by the Type-4 Induced-Association BRET Assay.
Felce JH, MacRae A, Davis SJ
Biophysical journal 2019 Jan 8;116(1):31-41
Biophysical journal 2019 Jan 8;116(1):31-41
High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.
O'Malley J, Skylaki S, Iwabuchi KA, Chantzoura E, Ruetz T, Johnsson A, Tomlinson SR, Linnarsson S, Kaji K
Nature 2013 Jul 4;499(7456):88-91
Nature 2013 Jul 4;499(7456):88-91
T cell-signaling network analysis reveals distinct differences between CD28 and CD2 costimulation responses in various subsets and in the MAPK pathway between resting and activated regulatory T cells.
Kalland ME, Oberprieler NG, Vang T, Taskén K, Torgersen KM
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5233-45
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):5233-45
Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity.
Liang HE, Reinhardt RL, Bando JK, Sullivan BM, Ho IC, Locksley RM
Nature immunology 2011 Dec 4;13(1):58-66
Nature immunology 2011 Dec 4;13(1):58-66
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Kap YS, van Meurs M, van Driel N, Koopman G, Melief MJ, Brok HP, Laman JD, 't Hart BA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
Cytokine-secreting follicular T cells shape the antibody repertoire.
Reinhardt RL, Liang HE, Locksley RM
Nature immunology 2009 Apr;10(4):385-93
Nature immunology 2009 Apr;10(4):385-93
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.125 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.125 µg of Anti-Human CD2 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Type-4 BRET assay applied to known controls. ( A ) The mean Delta BRET eff of type-1 transmembrane protein controls using the FKBP 1 type-4 BRET assay. Only the known dimers CD28 and CD80 exhibited significantly nonzero Delta BRET eff when expressed as homomeric pairs. Dark gray bars indicate like-like pairs. ( B ) The mean Delta BRET eff of type-1 transmembrane protein controls using the FKBP 3 type-4 BRET assay. Delta BRET eff is larger for CD28 and CD80 than with the FKBP 1 approach. ( C ) A histogram of CD2 expression in HEK-293T cells transfected with 1 mu g of differently tagged forms of CD2, as measured by flow cytometry. ( D ) Confocal microscopy of CD28-GFP coexpressed with CD28-FKBP 1/3 in the presence and absence of AP20187. No clear GFP internalization was evident. ( E ) The mean Delta BRET eff of GPCRs of known stoichiometry using the FKBP 3 type-4 BRET assay. For all panels, bars indicate mean +- SD. Probability is indicated for difference from Delta BRET eff = 0; * p < 0.05, *** p < 0.005. All data are the result of n >= 3 independent experiments.
- Conjugate
- Biotin
