Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Flow cytometry [2]
- Other assay [2]
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- Product number
- 17-0029-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD2 Monoclonal Antibody (RPA-2.10), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.
- Antibody clone number
- RPA-2.10
- Concentration
- 5 µL/Test
Submitted references Logic-gated antibody pairs that selectively act on cells co-expressing two antigens.
A20 and ABIN-1 cooperate in balancing CBM complex-triggered NF-κB signaling in activated T cells.
Regulation of the endosomal SNX27-retromer by OTULIN.
Constraints on GPCR Heterodimerization Revealed by the Type-4 Induced-Association BRET Assay.
Molecular architecture and regulation of BCL10-MALT1 filaments.
LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.
Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells.
NF-κB essential modulator (NEMO) interaction with linear and lys-63 ubiquitin chains contributes to NF-κB activation.
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Oostindie SC, Rinaldi DA, Zom GG, Wester MJ, Paulet D, Al-Tamimi K, van der Meijden E, Scheick JR, Wilpshaar T, de Jong B, Hoff-van den Broek M, Grattan RM, Oosterhoff JJ, Vignau J, Verploegen S, Boross P, Beurskens FJ, Lidke DS, Schuurman J, de Jong RN
Nature biotechnology 2022 Oct;40(10):1509-1519
Nature biotechnology 2022 Oct;40(10):1509-1519
A20 and ABIN-1 cooperate in balancing CBM complex-triggered NF-κB signaling in activated T cells.
Yin H, Karayel O, Chao YY, Seeholzer T, Hamp I, Plettenburg O, Gehring T, Zielinski C, Mann M, Krappmann D
Cellular and molecular life sciences : CMLS 2022 Jan 31;79(2):112
Cellular and molecular life sciences : CMLS 2022 Jan 31;79(2):112
Regulation of the endosomal SNX27-retromer by OTULIN.
Stangl A, Elliott PR, Pinto-Fernandez A, Bonham S, Harrison L, Schaub A, Kutzner K, Keusekotten K, Pfluger PT, El Oualid F, Kessler BM, Komander D, Krappmann D
Nature communications 2019 Sep 20;10(1):4320
Nature communications 2019 Sep 20;10(1):4320
Constraints on GPCR Heterodimerization Revealed by the Type-4 Induced-Association BRET Assay.
Felce JH, MacRae A, Davis SJ
Biophysical journal 2019 Jan 8;116(1):31-41
Biophysical journal 2019 Jan 8;116(1):31-41
Molecular architecture and regulation of BCL10-MALT1 filaments.
Schlauderer F, Seeholzer T, Desfosses A, Gehring T, Strauss M, Hopfner KP, Gutsche I, Krappmann D, Lammens K
Nature communications 2018 Oct 2;9(1):4041
Nature communications 2018 Oct 2;9(1):4041
LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.
Lu XJ, Chen Q, Rong YJ, Yang GJ, Li CH, Xu NY, Yu CH, Wang HY, Zhang S, Shi YH, Chen J
Nature communications 2016 Sep 6;7:12719
Nature communications 2016 Sep 6;7:12719
Alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells.
Meininger I, Griesbach RA, Hu D, Gehring T, Seeholzer T, Bertossi A, Kranich J, Oeckinghaus A, Eitelhuber AC, Greczmiel U, Gewies A, Schmidt-Supprian M, Ruland J, Brocker T, Heissmeyer V, Heyd F, Krappmann D
Nature communications 2016 Apr 12;7:11292
Nature communications 2016 Apr 12;7:11292
NF-κB essential modulator (NEMO) interaction with linear and lys-63 ubiquitin chains contributes to NF-κB activation.
Hadian K, Griesbach RA, Dornauer S, Wanger TM, Nagel D, Metlitzky M, Beisker W, Schmidt-Supprian M, Krappmann D
The Journal of biological chemistry 2011 Jul 22;286(29):26107-17
The Journal of biological chemistry 2011 Jul 22;286(29):26107-17
A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
Kap YS, van Meurs M, van Driel N, Koopman G, Melief MJ, Brok HP, Laman JD, 't Hart BA
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 Dec;57(12):1159-67
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (open histogram) or Anti-Human CD2 APC (filled histogram). Cells in the lymphocyte gate were used for analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, eFluor 450 (Product # 48-0199-42) and Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (left) or CD2 Monoclonal Antibody, APC (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Type-4 BRET assay applied to known controls. ( A ) The mean Delta BRET eff of type-1 transmembrane protein controls using the FKBP 1 type-4 BRET assay. Only the known dimers CD28 and CD80 exhibited significantly nonzero Delta BRET eff when expressed as homomeric pairs. Dark gray bars indicate like-like pairs. ( B ) The mean Delta BRET eff of type-1 transmembrane protein controls using the FKBP 3 type-4 BRET assay. Delta BRET eff is larger for CD28 and CD80 than with the FKBP 1 approach. ( C ) A histogram of CD2 expression in HEK-293T cells transfected with 1 mu g of differently tagged forms of CD2, as measured by flow cytometry. ( D ) Confocal microscopy of CD28-GFP coexpressed with CD28-FKBP 1/3 in the presence and absence of AP20187. No clear GFP internalization was evident. ( E ) The mean Delta BRET eff of GPCRs of known stoichiometry using the FKBP 3 type-4 BRET assay. For all panels, bars indicate mean +- SD. Probability is indicated for difference from Delta BRET eff = 0; * p < 0.05, *** p < 0.005. All data are the result of n >= 3 independent experiments.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 8 OTULIN binding to SNX27 limits recycling of GLUT1 and SLC1A4 to the cell surface. a GLUT1 surface staining in mock, OTULIN WT and OTULIN DeltaETSL transfected U2OS cells was performed using GLUT1.RBD.GFP reagent on living cells. GLUT1 surface expression was analyzed in untransfected (DeltaCD2-low) and in transfected (DeltaCD2-high) cells by FACS (left). Relative GLUT1 surface expression was determined as depicted in the histogram and changes in median fluorescence intensity (MFI) were normalized to mock. Graphs represent the mean +- SD of three independent experiments. Two-tailed p -values: ns not significant, *** p