AF2377

antibody from Novus Biologicals

Targeting: S100A10 42C, ANX2LG, CAL1L, CLP11, P11

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2377

Provider

Novus Biologicals

Product name

Goat Polyclonal S100A10 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse S100A10 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human (rh) S100A10 is observed and less than 1% cross-reactivity with rhS100B and recombinant mouse S100A9 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Ahnak scaffolds p11/Anxa2 complex and L-type voltage-gated calcium channel and modulates depressive behavior.

Jin J, Bhatti DL, Lee KW, Medrihan L, Cheng J, Wei J, Zhong P, Yan Z, Kooiker C, Song C, Ahn JH, Obermair GJ, Lee A, Gresack J, Greengard P, Kim Y
Molecular psychiatry 2020 May;25(5):1035-1049

---

PSA-NCAM Colocalized with Cholecystokinin-Expressing Cells in the Hippocampus Is Involved in Mediating Antidepressant Efficacy.

Yamada J, Sato C, Konno K, Watanabe M, Jinno S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Jan 22;40(4):825-842

---

P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice.

Liu G, Wang Y, Zheng W, Cheng H, Zhou R
International journal of biological sciences 2019;15(7):1383-1395

---

Gestational stress in mouse dams negatively affects gestation and postpartum hippocampal BDNF and P11 protein levels.

Vanmierlo T, De Vry J, Nelissen E, Sierksma A, Roumans N, Steinbusch HWM, Wennogle LP, van den Hove D, Prickaerts J
Molecular and cellular neurosciences 2018 Apr;88:292-299

---

Molecular codes for cell type specification in Brn3 retinal ganglion cells.

Sajgo S, Ghinia MG, Brooks M, Kretschmer F, Chuang K, Hiriyanna S, Wu Z, Popescu O, Badea TC
Proceedings of the National Academy of Sciences of the United States of America 2017 May 16;114(20):E3974-E3983

---

Identification of the cortical neurons that mediate antidepressant responses.

Schmidt EF, Warner-Schmidt JL, Otopalik BG, Pickett SB, Greengard P, Heintz N
Cell 2012 May 25;149(5):1152-63

---

Characterization of S100A11, a suppressive factor of fertilization, in the mouse female reproductive tract.

Hanaue M, Miwa N, Uebi T, Fukuda Y, Katagiri Y, Takamatsu K
Molecular reproduction and development 2011 Feb;78(2):91-103

---

Role of p11 in cellular and behavioral effects of 5-HT4 receptor stimulation.

Warner-Schmidt JL, Flajolet M, Maller A, Chen EY, Qi H, Svenningsson P, Greengard P
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Feb 11;29(6):1937-46

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse S100A10 by Simple Western<SUP abp="264">TM. Simple Western lane view shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells, loaded at 0.2 mg/mL. A specific band was detected for S100A10 at approximately 11 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse S100A10 by Western Blot. Western blot shows lysates of mouse lung and MEF mouse embryonic feeder cells. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

S100A10 in Mouse Kidney. S100A10 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.