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LearnPA5-78146
antibody from Invitrogen Antibodies
Targeting: RAP1GAP
KIAA0474, RAP1GA1, RAP1GAP1, RAP1GAPII
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-78146 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAP1GAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: mouse brain, rat brain
- Concentration
- 1.7 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAP1GAP in various tissue extracts using 50 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with RAP1GAP polyclonal antibody (Product # PA5-78146) using a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAP1GAP Polyclonal Antibody (Product # PA5-78146). Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RAP1GAP Polyclonal Antibody (Product # PA5-78146) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAP1GAP Polyclonal Antibody detects Rap1GAP protein at Golgi apparatus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in ice-cold MeOH for 5 min. Green: Rap1GAP protein stained by RAP1GAP Polyclonal Antibody (Product # PA5-78146) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
