Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP2-61940 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Follistatin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody recognizes human Follistatin isoform FS315.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Follistatin Antibody ([H10]) - Azide and BSA Free [NBP2-61940] - Western Blots showing binding of clone H10 to isoforms of FS315 in prostate tumor cell lines. B. FS315 proteins corresponding to 35, 39, and 42K molecular weight isoforms of FS could only be detected by the H10 Ab in lanes containing hrFS315 (lane 3).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Follistatin Antibody ([H10]) - Azide and BSA Free [NBP2-61940] - Clone H10 used to detect immunoreactivity with FS315 by IHC-P. Immunolocalization of FS315 and FS288 to PC3 and LNCaP cell lines. Positive immunoreactivity for FS315 localized to the cytoplasm of the epithelial tumor cell lines LNCaP (A) and PC3 (B) using H10 antibody; no immunoreactivity was present if the antibody was preabsorbed with FS315 peptide (inset A, inset B). Mouse IgG antibody controls were negative (inset C, inset D).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Follistatin Antibody ([H10]) - Azide and BSA Free [NBP2-61940] - Clone H10 used to detect expression of FS315 in TM cells by IHC-P. FST 315 expression in NTM (C) and GTM (G) tissues.