AF2585
antibody from R&D Systems
Targeting: CSPG4
CSPG4A, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF2585 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NG2/MCSP Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human NG2/MCSP in direct ELISAs and Western blots. In Western blots, approximately 30% cross-reactivity with recombinant mouse CSPG4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q6UVK1
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IGFBP7, a novel tumor stroma marker, with growth-promoting effects in colon cancer through a paracrine tumor-stroma interaction.
Rupp C, Scherzer M, Rudisch A, Unger C, Haslinger C, Schweifer N, Artaker M, Nivarthi H, Moriggl R, Hengstschläger M, Kerjaschki D, Sommergruber W, Dolznig H, Garin-Chesa P
Oncogene 2015 Feb 12;34(7):815-25
Oncogene 2015 Feb 12;34(7):815-25
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human NG2/MCSP by Western Blot. Western blot shows lysates of A375 human melanoma cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human NG2/MCSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2585) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human NG2/MCSP Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human NG2/MCSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2585) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.