GTX129464

antibody from GeneTex

Targeting: HMGB2 HMG2

Antibody data

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Validation data

HMGB2 protein structure - GTX129464 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody (GTX129464) diluted at 1:2000.
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 41486

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HMGB2 antibody detects HMGB2 protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract12 % SDS-PAGEHMGB2 antibody (GTX129464) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HMGB2 antibody detects HMGB2 protein by western blot analysis. Non-transfected (-), HMGB1-transfected (+) and HMGB2-transfected (+) whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody (GTX129464) diluted at 1:3000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody (GTX129464) diluted at 1:2000.