PA5-33389

antibody from Invitrogen Antibodies

Targeting: CCKBR

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-33389

Provider

Invitrogen Antibodies

Product name

CCKBR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon (100%) Monkey (83%).

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

50 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Expression of the Cholecystokinin-B Receptor in Neoplastic Gastric Cells.

Mjønes P, Nordrum IS, Sørdal Ø, Sagatun L, Fossmark R, Sandvik A, Waldum HL
Hormones & cancer 2018 Feb;9(1):40-54

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed paraffin-embedded human stomach using a Cckb/CCKBR polyclonal antibody (Product # PA5-33389). Heat-induced antigen retrieval was performed prior to staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 Case 6, neuroendocrine tumor grade 1 in a patient with chronic atrophic gastritis illustrated by a Hematoxylin and eosin, x 20, and by positive expression for b chromogranin A, x 20, c synaptophysin, x 20, d cholecystokinin B receptor, x 20, e in situ hybridization with two probes, illustrating co-expression of cholecystokinin B receptor (green/blue) and chromogranin A (red), x 40, f in situ hybridization with dual expression of cholecystokinin B receptor and chromogranin A in neuroendocrine cells (blue/green = cholecystokinin B receptor, red = chromogranin A, black = dual expression), x 40

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 Case 7, patient with enterochromaffin-like cell hyperplasia, neuroendocrine tumor and neuroendocrine carcinoma illustrated by, a hematoxylin and eosin, neuroendocrine tumor, x 20, b hematoxylin and eosin, neuroendocrine carcinoma, x 20, c chromogranin A positive neuroendocrine tumor, x 20, d chromogranin A negative neuroendocrine carcinoma, x 20, e synaptophysin positive neuroendocrine tumor, x 20, f synaptophysin positive neuroendocrine carcinoma, x 20, g Ki67 expression in 2-5% of tumor cells, neuroendocrine tumor, x 20, h Ki67 expression in > 20% of tumor cells, neuroendocrine carcinoma, x 20, i ) cholecystokinin-B receptor protein expression, neuroendocrine tumor, x 20, j ) cholecystokinin-B receptor protein expression, neuroendocrine carcinoma x 20, k ) in situ hybridization, cells with dual expression of cholecystokinin-B receptor and chromogranin A in neuroendocrine carcinoma (red/black/green), x 40. Areas of co-localization appear black

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 Normal gastric mucosa from a patient with Zollinger-Ellison syndrome illustrated by a hematoxylin and eosin, x 10, b chromogranin A (protein) positive neuroendocrine cells, x 10, c cholecystokinin-B receptor (protein) expression in normal gastric mucosa, x 10, and d in situ hybridization (duplex) illustrating dual expression of cholecystokinin-B receptor mRNA (green/blue) and chromogranin A mRNA (red) in neuroendocrine cell (dual expression appearing black), and cholecystokinin-B receptor positive cells in other epithelial cells below the isthmus of the gastric gland