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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AP3443a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP3443a, RRID:AB_1278213
- Product name
- Phospho-RAF1(S259) Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.25 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Calcineurin increases glucose activation of ERK1/2 by reversing negative feedback.
Duan L, Cobb MH
Proceedings of the National Academy of Sciences of the United States of America 2010 Dec 21;107(51):22314-9
Proceedings of the National Academy of Sciences of the United States of America 2010 Dec 21;107(51):22314-9
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Supportive validation
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- Abgent (provider)
- Main image
- Experimental details
- Dot blot analysis of anti-RAF1-pS259 Phospho-specific Pab (RB11131) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
- Primary Ab dilution
- 1:500
