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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-37677 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-PAK1 (Thr212) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is 293 cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references MicroRNA-28-5p inhibits the migration and invasion of gastric cancer cells by suppressing AKT phosphorylation.
Xiao F, Cheng Z, Wang P, Gong B, Huang H, Xing Y, Liu F
Oncology letters 2018 Jun;15(6):9777-9785
Oncology letters 2018 Jun;15(6):9777-9785
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from 293 cells, untreated or treated with forskolin, using PAK1 (pThr212) polyclonal antibody (Product # PA5-37677).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-PAK1 (Thr212) in methanol-fixed Hela cells, using Phospho-PAK1 (Thr212) Polyclonal Antibody (Product # PA5-37677).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-PAK1 (Thr212) in paraffin-embedded Human breast carcinoma tissue using Phospho-PAK1 (Thr212) Polyclonal Antibody (Product # PA5-37677) (left) or the same antibody preincubated with blocking peptide (right).
