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Western blotAntibody data
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- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX128155 - Provider product page
- Provider
- GeneTex
- Product name
- CDC2 (phospho Tyr15) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references The application of flow cytometry for evaluating biological aggressiveness of intracranial meningiomas.
Lin YW, Tai SH, Huang YH, Chang CC, Juan WS, Chao LC, Wen MJ, Hung YC, Lee EJ
Cytometry. Part B, Clinical cytometry 2015 Sep-Oct;88(5):312-9
Cytometry. Part B, Clinical cytometry 2015 Sep-Oct;88(5):312-9
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- CDC2 (phospho Tyr15) antibody detects CDK1 protein by Western blot analysis.A. 30 ?g HeLa whole cell lysate/extract (untreated) B. 30 ?g HeLa whole cell lysate/extract (2mM double thymidine treatment and recovery for 3.5hr) C. 30 ?g HeLa whole cell lysate/extract (2mM double thymidine treatment and recovery for 12hr)10 % SDS-PAGECDC2 (phospho Tyr15) antibody (GTX128155) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDC2 (phospho Tyr15) antibody detects CDK1 protein by Western blot analysis.A. 30 ?g HeLa whole cell lysate/extract (untreated) B. 30 ?g HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)10 % SDS-PAGECDC2 (phospho Tyr15) antibody (GTX128155) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of CDC2 (phospho Tyr15) protein from HeLa whole cell extracts using 5 £gg of CDC2 (phospho Tyr15) antibody (GTX128155).Western blot analysis was performed using CDC2 (phospho Tyr15) antibody (GTX128155).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDC2 (phospho Tyr15) antibody detects CDC2 (phospho Tyr15) protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. CDC2 (phospho Tyr15) antibody (GTX128155) diluted at 1:500.
