MA5-15400

antibody from Invitrogen Antibodies

Targeting: IGFBP2 IBP2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15400

Provider

Invitrogen Antibodies

Product name

IGFBP2 Monoclonal Antibody (1F6F6)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15400 targets IGFBP2 in IHC and WB applications and shows reactivity with Human samples.

Antibody clone number

1F6F6

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IGFBP2 using a IGFBP2 monoclonal antibody (Product # MA5-15400) against a truncated IGFBP2-His recombinant protein (1) and truncated IGFBP2 (aa40-328) human IgG Fc transfected CHO-K1 cell lysate (2).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-IGFBP2 Monoclonal Antibody (1F6F6), (Product # MA5-15400) and a 35 kDa band corresponding to IGFBP2 was observed in the cell lines tested and increased upon protein transport inhibitor in SH-SY5Y. Membrane enriched cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y treated with Protein Transport Inhibitor (1X for 4 hours) (Lane 2), OVCAR-3 (Lane 3), and SK-OV-3 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung cancer (A), rectum (B), prostate (C), colon cancer (D) showing cytoplasmic localization using IGFBP2 monoclonal antibody (Product # MA5-15400) followed with DAB staining.