PA5-46940

antibody from Invitrogen Antibodies

Targeting: CCL19 CKb11, ELC, exodus-3, MIP-3b, SCYA19

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46940

Provider

Invitrogen Antibodies

Product name

MIP-3 beta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) MIP-3 beta, recombinant human (rh) GRO alpha, rhHCC-4, rhEotaxin, rmMIP-3 alpha, recombinant rat MIP-3 alpha, and rhMIP-3 alpha is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MIP-3 beta in conditioned media from monocyte-derived macrophages . Samples were incubated in MIP-3 beta polyclonal antibody (Product # PA5-46940) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. Untreated (-) or treated (+) with 1 μg/mL LPS and 40 ng/mL Recombinant Human IFN‚γ for 24 hours. A specific band was detected for CCL19/MIP‚3β at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CCL19 in conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 μg/mL LPS and 40 ng/mL Recombinant Human IFN‚γ. Samples were incubated in CCL19 polyclonal antibody (Product # PA5-46940) using a dilution of 50 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 0.0763888888888889. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of MIP-3 beta in BaF3 mouse pro‚B cell line transfected with human CCR7. Samples were incubated in MIP-3 beta polyclonal antibody (Product # PA5-46940). Recombinant Human CCL19/MIP‚3β chemoattracts the BaF3 mouse pro‚B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL19/MIP‚3β (50 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL19/MIP‚3β Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 2-10 µg/mL.