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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- AP09358PU-S - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-UBE2J1 Antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-UBE2J1 Antibody, Aff - Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25ul
- Concentration
- 0.71 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-Ube2j1 antibody shows detection of Ube2j1 in 293 cells overexpressing Myc-Ube2j1 (Lane 2). Lane 1 contains lysate from mocktransfected 293 cells.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a = amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thioesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein)/CBC(cullin-2/elonginB/elonginC)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.
- Validation comment
- Assay
