Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA800055 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA800055, RRID:AB_2625182
- Product name
- UBE2E3 mouse monoclonal antibody, clone OTI4C8 (formerly 4C8)
- Antibody type
- Monoclonal
- Description
- UBE2E3 mouse monoclonal antibody, clone OTI4C8 (formerly 4C8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- UBE2E3
- Isotype
- IgG
- Antibody clone number
- OTI4C8
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2E3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3 (RC210328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2E3.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-UBE2E3 mouse monoclonal antibody (TA800055) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2E3(RC210328).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-UBE2E3 antibody(TA800055),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
