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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- TA800046 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA800046, RRID:AB_2625173
- Product name
- UBE2E3 mouse monoclonal antibody, clone OTI3E5 (formerly 3E5)
- Antibody type
- Monoclonal
- Description
- UBE2E3 mouse monoclonal antibody, clone OTI3E5 (formerly 3E5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- UBE2E3
- Isotype
- IgG
- Antibody clone number
- OTI3E5
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3 (RC210328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2E3.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2E3 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC210328 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UBE2E3 antibody(TA800046), and then analyzed by flow cytometry.
- Validation comment
- FC
