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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-31239 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JTV-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HL-60.
- Concentration
- 0.84 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using JTV-1 Polyclonal Antibody (Product # PA5-31239). Sample (30 µg of whole cell lysate). Lane A: HL-60. 10% SDS PAGE. JTV-1 Polyclonal Antibody (Product # PA5-31239) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AIMP2 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using an AIMP2 polyclonal antibody (Product # PA5-31239) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- JTV-1 Polyclonal Antibody detects AIMP2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AIMP2 protein stained by JTV-1 Polyclonal Antibody (Product # PA5-31239) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- JTV-1 Polyclonal Antibody detects AIMP2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AIMP2 protein stained by JTV-1 Polyclonal Antibody (Product # PA5-31239) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- JTV-1 Polyclonal Antibody detects AIMP2 protein at cytoplasm on human normal kidney by immunohistochemical analysis. Sample: Paraffin-embedded human normal kidney. JTV-1 Polyclonal Antibody (Product # PA5-31239) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
