A21980

antibody from Invitrogen Antibodies

Targeting: ACADVL ACAD6, LCACD, VLCAD

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A21980

Provider

Invitrogen Antibodies

Product name

Anti-ACADVL Monoclonal Antibody (6A9AF2)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat, Bovine

Host

Mouse

Isotype

IgG

Antibody clone number

6A9AF2

Vial size

100 µg

Concentration

1 mg/ml

Storage

4° C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human HDFn cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody at 5 µg/mL overnight at 4°C. Secondary antibody: (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical localization of mitochondrial and metabolic enzymes in tissue sections of normal, aged human cerebellar tissue (formalin fixed paraffin-embedded). Immunolabeling was carried out with primary antibodies diluted in TBST/4% BSA at room temperature for one hour. Immunodetection was carried out using a commercial HRP Detection Kit according the manufacturer’s instructions.