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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-40584 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MLLT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: EESNSEDEAS FKSESAQSSP SNSSSSSDSS SDSDFEPSQN HSQGPLRSMV
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MLLT1 (Ear in Drosophila) using MLLT1 Rabbit Polyclonal Antibody (Product # PA5-40584) was performed by loading 10 mg of each cell lysates: HeLa (lane 1) and Drosophila S2 (lane 2) in sample buffer and 5 µL Spectra Multicolor Broad Range Protein Ladder (Product # 26634) onto a 10% SDS-PAGE gel. Proteins were transferred to PVDF membrane (Product # 88518) at 30V for 60 min on ice. Membrane was blocked in 5% Milk in PBST for 30 min at room temperature. The primary polyclonal MLLIT1 antibody (Product # PA5-40584) was used at a dilution of 1:500 in 5% Milk in PBST overnight at 4ºC. Followed by goat anti-rabbit alkaline phosphatase secondary antibody at a dilution of 1:20,000 for 1 hr. Chemiluminescent detection was performed using ECL substrate. Data courtesy of Dr. Fondufe-Mittendorf’s lab at University of Kentucky.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MLLT1 (Ear in Drosophila) using MLLT1 Rabbit Polyclonal Antibody (Product # PA5-40584) was performed by loading 10 mg of each cell lysates: HeLa (lane 1) and Drosophila S2 (lane 2) in sample buffer and 5 µL Spectra Multicolor Broad Range Protein Ladder (Product # 26634) onto a 10% SDS-PAGE gel. Proteins were transferred to PVDF membrane (Product # 88518) at 30V for 60 min on ice. Membrane was blocked in 5% Milk in PBST for 30 min at room temperature. The primary polyclonal MLLIT1 antibody (Product # PA5-40584) was used at a dilution of 1:500 in 5% Milk in PBST overnight at 4ºC. Followed by goat anti-rabbit alkaline phosphatase secondary antibody at a dilution of 1:20,000 for 1 hr. Chemiluminescent detection was performed using ECL substrate. Data courtesy of Dr. Fondufe-Mittendorf’s lab at University of Kentucky.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Raji cell lysate using an anti-MLLT1 polyclonal antibody (Product # PA5-40584).
