PA5-80397

antibody from Invitrogen Antibodies

Targeting: CA8 CALS, CARP

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80397

Provider

Invitrogen Antibodies

Product name

Carbonic Anhydrase VIII Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Carbonic Anhydrase VIII in Lane A: A549 Membrane Lysate (30 µg). Samples were probed using a Carbonic Anhydrase VIII Polyclonal Antibody (Product # PA5-80397) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:33 kDa. Observed band size:38 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Carbonic Anhydrase VIII Polyclonal Antibody (Product # PA5-80397) at 1:500 dilution. Lane A: A549 Membrane Lysate. Lysates/proteins at 30 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 38 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Carbonic Anhydrase VIII Immunoprecipitation using: Lane A: 0.5 mg A549 Whole Cell Lysate 2 µL with Carbonic Anhydrase VIII Polyclonal Antibody (Product # PA5-80397) and 15 µL of 50 % Protein G agarose. Primary antibody: Carbonic Anhydrase VIII Polyclonal Antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 37 kDa.