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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
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- Product number
- GTX129710 - Provider product page
- Provider
- GeneTex
- Product name
- GABARAP antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX129710) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GABARAP antibody detects GABARAP protein by western blot analysis.A. 30 £gg Jurkat whole cell lysate/extract B. 30 £gg Raji whole cell lysate/extract C. 30 £gg NCI-H929 whole cell lysate/extract15 % SDS-PAGEGABARAP antibody (GTX129710) dilution: 1:2000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GABARAP antibody detects GABARAP protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract 15 % SDS-PAGEGABARAP antibody (GTX129710) dilution: 1:2000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GABARAP antibody detects GABARAP protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 whole cell lysate/extract of human GABARAP -transfected 293T cells15 % SDS-PAGEGABARAP antibody (GTX129710) dilution: 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAP antibody (GTX129710) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GABARAP antibody detects GABARAP protein at cytoplasm by immunofluorescent analysis.Sample: U2OS cells were fixed in ice-cold MeOH for 5 min.Green: GABARAP protein stained by GABARAP antibody (GTX129710) diluted at 1:500.Blue: Hoechst 33342 staining.
