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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX112927 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112927, RRID:AB_10722802
- Product name
- Estrogen Receptor beta antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Submitted references Exposure to the polyester PET precursor--terephthalic acid induces and perpetuates DNA damage-harboring non-malignant human breast cells.
Luciani-Torres MG, Moore DH, Goodson WH 3rd, Dairkee SH
Carcinogenesis 2015 Jan;36(1):168-76
Carcinogenesis 2015 Jan;36(1):168-76
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 μg of whole cell lysate) A: NT2D1 7.5% SDS PAGE GTX112927 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Estrogen Receptor beta antibody detects Estrogen Receptor beta protein by western blot analysis.A. 30 μg MCF-7 whole cell lysate/extract B. 30 μg MDA-MB-231 whole cell lysate/extract7.5 % SDS-PAGEEstrogen Receptor beta antibody (GTX112927) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Estrogen Receptor beta antibody detects Estrogen Receptor beta protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Estrogen Receptor beta protein stained by Estrogen Receptor beta antibody (GTX112927) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Estrogen Receptor beta antibody detects Estrogen Receptor beta protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Estrogen Receptor beta antibody (GTX112927) diluted at 1:500.
