NBP2-61942

antibody from Novus Biologicals

Targeting: ESR2 ER-beta, Erb, NR3A2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-61942

Provider

Novus Biologicals

Product name

Mouse Monoclonal ER beta/NR3A2 Antibody

Antibody type

Monoclonal

Description

Protein A purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ER beta/NR3A2 Antibody ([PPG5/25]) - Azide and BSA Free [NBP2-61942] - Clone PPG5/25 used to detect ER beta/NR3A2 mRNA expression in colorectal carcinoma cells by Western Blot. (A) ER beta 1, 2 and ER beta/NR3A2 mRNA expression (assessed by RT-PCR) and (B) ER beta 1 and 2 protein expression (assessed by western blotting) by 10 colorectal carcinoma cell lines.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: ER beta/NR3A2 Antibody ([PPG5/25]) - Azide and BSA Free [NBP2-61942] - Clone PPG5/25 used to detect expression of ER beta/NR3A2 in cancer tissue by IHC-P. Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: ER beta/NR3A2 Antibody ([PPG5/25]) - Azide and BSA Free [NBP2-61942] - Clone PPG5/25 used to detect expression of ER beta/NR3A2 in cancer tissue by IHC-P. A) Immunostaining for ER beta 1 was significantly higher in tumors from females compared to males (p

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: ER beta/NR3A2 Antibody ([PPG5/25]) - Azide and BSA Free [NBP2-61942] - Clone PPG5/25 used to detect ER beta/NR3A2 expression in normal human liver by IHC-P. Staining pattern of the ER beta isoforms in human liver. In order to confirm the specificity of the ER beta antibodies,, sections of healthy human liver were immunostained, showing that ER beta 1 and ER beta 2 isoforms are not expressed, while specific nuclear ER beta 5 immunoreactivity is detected (magnification 20x and 40x). This pattern of ER beta isoforms' immunoreactivity is consistent with previous descriptions.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: ER beta/NR3A2 Antibody ([PPG5/25]) - Azide and BSA Free [NBP2-61942] - Clone PPG5/25 used to detect expression of ER beta/NR3A2 in colorectal tissue by IHC-P. Examples of ER beta 1 (a, d, e), ER beta 2 (b, f, g), ER beta 2 (c, h) and TFF1 (i) immunohistochemistry performed on normal colorectal epithelium (a-c) and colorectal carcinoma (d-i).