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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- NBP1-41194 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-41194, RRID:AB_2231561
- Product name
- Mouse Monoclonal ER beta/NR3A2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody will recognize human ER beta.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Estrogen receptor beta binds to and regulates three distinct classes of target genes.
Genome-wide dynamics of chromatin binding of estrogen receptors alpha and beta: mutual restriction and competitive site selection.
Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethane.
Estrogen Receptors alpha and beta as determinants of gene expression: influence of ligand, dose, and chromatin binding.
Selective activation of estrogen receptor-beta transcriptional pathways by an herbal extract.
Distinct roles of unliganded and liganded estrogen receptors in transcriptional repression.
Estradiol and selective estrogen receptor modulators differentially regulate target genes with estrogen receptors alpha and beta.
Functional implications of antiestrogen induction of quinone reductase: inhibition of estrogen-induced deoxyribonucleic acid damage.
Reciprocal recruitment of DRIP/mediator and p160 coactivator complexes in vivo by estrogen receptor.
Vivar OI, Zhao X, Saunier EF, Griffin C, Mayba OS, Tagliaferri M, Cohen I, Speed TP, Leitman DC
The Journal of biological chemistry 2010 Jul 16;285(29):22059-66
The Journal of biological chemistry 2010 Jul 16;285(29):22059-66
Genome-wide dynamics of chromatin binding of estrogen receptors alpha and beta: mutual restriction and competitive site selection.
Charn TH, Liu ET, Chang EC, Lee YK, Katzenellenbogen JA, Katzenellenbogen BS
Molecular endocrinology (Baltimore, Md.) 2010 Jan;24(1):47-59
Molecular endocrinology (Baltimore, Md.) 2010 Jan;24(1):47-59
Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethane.
Vivar OI, Saunier EF, Leitman DC, Firestone GL, Bjeldanes LF
Endocrinology 2010 Apr;151(4):1662-7
Endocrinology 2010 Apr;151(4):1662-7
Estrogen Receptors alpha and beta as determinants of gene expression: influence of ligand, dose, and chromatin binding.
Chang EC, Charn TH, Park SH, Helferich WG, Komm B, Katzenellenbogen JA, Katzenellenbogen BS
Molecular endocrinology (Baltimore, Md.) 2008 May;22(5):1032-43
Molecular endocrinology (Baltimore, Md.) 2008 May;22(5):1032-43
Selective activation of estrogen receptor-beta transcriptional pathways by an herbal extract.
Cvoro A, Paruthiyil S, Jones JO, Tzagarakis-Foster C, Clegg NJ, Tatomer D, Medina RT, Tagliaferri M, Schaufele F, Scanlan TS, Diamond MI, Cohen I, Leitman DC
Endocrinology 2007 Feb;148(2):538-47
Endocrinology 2007 Feb;148(2):538-47
Distinct roles of unliganded and liganded estrogen receptors in transcriptional repression.
Cvoro A, Tzagarakis-Foster C, Tatomer D, Paruthiyil S, Fox MS, Leitman DC
Molecular cell 2006 Feb 17;21(4):555-64
Molecular cell 2006 Feb 17;21(4):555-64
Estradiol and selective estrogen receptor modulators differentially regulate target genes with estrogen receptors alpha and beta.
Tee MK, Rogatsky I, Tzagarakis-Foster C, Cvoro A, An J, Christy RJ, Yamamoto KR, Leitman DC
Molecular biology of the cell 2004 Mar;15(3):1262-72
Molecular biology of the cell 2004 Mar;15(3):1262-72
Functional implications of antiestrogen induction of quinone reductase: inhibition of estrogen-induced deoxyribonucleic acid damage.
Bianco NR, Perry G, Smith MA, Templeton DJ, Montano MM
Molecular endocrinology (Baltimore, Md.) 2003 Jul;17(7):1344-55
Molecular endocrinology (Baltimore, Md.) 2003 Jul;17(7):1344-55
Reciprocal recruitment of DRIP/mediator and p160 coactivator complexes in vivo by estrogen receptor.
Burakov D, Crofts LA, Chang CP, Freedman LP
The Journal of biological chemistry 2002 Apr 26;277(17):14359-62
The Journal of biological chemistry 2002 Apr 26;277(17):14359-62
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: ER beta/NR3A2 Antibody (7B10.7) [NBP1-41194] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [7B10.7]. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
