NB300-747

antibody from Novus Biologicals

Targeting: ESR2 ER-beta, Erb, NR3A2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-747

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-747, RRID:AB_2102251

Product name

Rabbit Polyclonal ER beta/NR3A2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects estrogen receptor (ER) beta from human and rat tissues. Detects a band at ~ 55 kDa.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

50ug

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

AKT regulation of estrogen receptor beta transcriptional activity in breast cancer.

Duong BN, Elliott S, Frigo DE, Melnik LI, Vanhoy L, Tomchuck S, Lebeau HP, David O, Beckman BS, Alam J, Bratton MR, McLachlan JA, Burow ME
Cancer research 2006 Sep 1;66(17):8373-81

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ER beta/NR3A2 Antibody [NB300-747] - Analysis of ER beta was performed by loading the indicated amounts of recombinant full-length human ER alpha and ER beta proteins, and 10ul of PageRuler Prestained Protein Ladder per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Blotter, and blocked with 5% milk in TBST for at least 1 hour at room temp. ER beta was detected at ~54kD (left panel) using an ER beta polyclonal antibody at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000. Chemiluminescent detection was performed using SuperSignal West Pico. The blot was then stripped with Restore Plus Western blot stripping buffer for at least 15 min at room temp, re-blocked with 5% milk in TBST for at least 1 hour at room temp, and re-probed with an ER alpha polyclonal antibody at a dilution of 1:2000 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000. Chemiluminescent detection was performed using SuperSignal West Pico. The presence of faint ER-beta bands on the blot probed.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: ER beta/NR3A2 Antibody [NB300-747] - Staining of rat prostate.