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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB664A - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal LIGHT/TNFSF14 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human LIGHT/TNFSF14 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) Fas Ligand, rhAPRIL, recombinant mouse TRANCE, rhTNF-alpha or rhTRAIL is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
Holmes TD, Wilson EB, Black EV, Benest AV, Vaz C, Tan B, Tanavde VM, Cook GP
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 30;111(52):E5688-96
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 30;111(52):E5688-96
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of LIGHT/TNFSF14 in Human CD3+ T cells by Flow Cytometry. Human CD3+ T cells treated with 1 ng/mL PMA and Calcium Ionomycin for 36 hours were stained with Mouse Anti-Human LIGHT/TNFSF14 APC-conjugated Monoclonal Antibody (Catalog # FAB664A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). Cells were gate on CD4+ cells using Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). View our protocol for Staining Membrane-associated Proteins.
