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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NB600-889 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-889, RRID:AB_10001370
- Product name
- Rabbit Polyclonal CDC16 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the phosphorylated form of APC6 at the T580 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.97 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: CDC16 [p Thr580] Antibody [NB600-889] - Analysis of a band 72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
