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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-23816 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD122 Monoclonal Antibody (27302)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R alpha, rh gamma c, rhIL-4 R, or rhIL-6 R is observed.
- Antibody clone number
- 27302
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD122 in immersion fixed frozen sections of human lymph node. Samples were incubated in CD122 monoclonal antibody (Product # MA5-23816) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of CD122 in MO7e human megakaryocytic leukemic cell line. Samples were incubated in CD122 monoclonal antibody (Product # MA5-23816). Recombinant Human IL‚2 stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‚2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‚2 Rβ Monoclonal Antibody. The ND50 is typically 0.05‚0.15 µg/mL.
