PA5-19976

antibody from Invitrogen Antibodies

Targeting: TNFSF13 APRIL, CD256

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19976

Provider

Invitrogen Antibodies

Product name

APRIL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

In Western blot applications, this antibody detects a band at ~27kDa. A suggested positive control is Jurkat cell lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Jurkat cells using a CD256/APRIL polyclonal antibody (Product # PA5-19976) at 1 µg/mL (A), 2 µg/mL (B), and 4 µg/mL (C).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of APRIL expression in Jurkat cells with APRIL Polyclonal Antibody (Product # PA5-19976) at 1 µg/mL (A), 2 µg/mL (B), and 4 µg/mL (C).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-APRIL Polyclonal Antibody (Product # PA5-19976) and 38 kDa and 25 kDa band corresponding to two isoforms of Tumor necrosis factor ligand superfamily member 13 were observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HCT 116 (Lane 1), HT-29 (Lane 2), SW480 (Lane 3), HUVEC (Lane 4), HeLa (Lane 5), Jurkat (Lane 6), Mouse Spleen (Lane 7) and Mouse Heart (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of APRIL in human prostate tissue with APRIL Polyclonal Antibody (Product # PA5-19976) at 10 µg/mL.