699002

antibody from BioLegend

Targeting: LAT2 HSPC046, LAB, NTAL, WBSCR15, WBSCR5, WSCR5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

699002

Provider

BioLegend

Proper citation

BioLegend Cat#699002, RRID:AB_2715812

Product name

Purified anti-NTAL (LAT2)

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

W15102A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates from Raw264.7 (negative control, Lane 1), HEK293T (negative control, Lane 2), Daudi (Lane 3) , and THP-1 (Lane 4) cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1µg/mL (1:500) purified anti-NTAL (LAT2) clone 15102A (left) antibody and a competitor’s clone used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (cat #405301) for clone W15102A, and HRP donkey anti-rabbit IgG (cat #406401) for the competitor’s clone, each at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat no. 643807) was used as a loading control at a 1:2000 dilution (lower). Lane M: Molecular weight ladder.

Conjugate

Unconjugated