PA5-54682

antibody from Invitrogen Antibodies

Targeting: ADHFE1 FLJ32430

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-54682

Provider

Invitrogen Antibodies

Product name

ADHFE1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LEMAEILGAD TRTARIQDAG LVLADTLRKF LFDLDVDDGL AAVGYSKADI PALVKGTLPQ ERVTKLAPCP QSEEDLAALF EASMKL

Concentration

0.6 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ADHFE1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an ADHFE1 Polyclonal Antibody (Product # PA5-54682).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ADHFE1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an ADHFE1 Polyclonal Antibody (Product # PA5-54682).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ADHFE1 in human cell line A-431 shows positivity in vesicles. Samples were probed using an ADHFE1 Polyclonal Antibody (Product # PA5-54682).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADHFE1 in human liver tissue shows cytoplasmic positivity in hepatocytes. Samples were probed using an ADHFE1 Polyclonal Antibody (Product # PA5-54682).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADHFE1 in human liver and pancreas tissues using ADHFE1 Polyclonal Antibody (Product # PA5-54682). Corresponding ADHFE1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADHFE1 in human pancreas using ADHFE1 Polyclonal Antibody (Product # PA5-54682) shows low expression as expected.