AF3268

antibody from Novus Biologicals

Targeting: CSF1R C-FMS, CD115, CSFR, FMS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

AF3268

Provider

Novus Biologicals

Product name

Rabbit Polyclonal M-CSFR/CD115 Antibody

Antibody type

Polyclonal

Description

Antigen and protein A Affinity-purified. Detects human M-CSF R/CD115 when phosphorylated at Y723 in Western blots.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Phospho-M-CSF R/CD115 (Y723) by Western Blot. Western blot shows lysates of M-CSF R/CD115-transfected NS0 mouse myeloma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human M-CSF/CD115 (Catalog # 216-MC) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-M-CSF R/CD115 (Y723) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3268), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-M-CSF R/CD115 (Y723) at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.