Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB3291-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal M-CSFR/CD115 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human M-CSF R/CD115 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM-CSF R beta is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human M-CSF R/CD115 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for M-CSF R/CD115 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- M-CSF R/CD115 Inhibition of M-CSF-dependent Cell Proliferation and Neutralization by Human M-CSF R/CD115 Antibody. Recombinant Human M-CSF R/CD115 Fc Chimera (Catalog # 329-MR) inhibits Recombinant Human M-CSF (Catalog # 216-MC) induced proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human M-CSF R/CD115 (1 ng/mL) activity elicited by Recombinant Human M-CSF R/CD115 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291). The ND50 is typically 4-16 ng/mL.