Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF3818 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse M-CSF R/CD115 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse M-CSF R/CD115 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human M-CSF R/CD115 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P09581
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Essential roles for the Tec family kinases Tec and Btk in M-CSF receptor signaling pathways that regulate macrophage survival.
Essential roles for the Tec family kinases Tec and Btk in M-CSF receptor signaling pathways that regulate macrophage survival.
Melcher M, Unger B, Schmidt U, Rajantie IA, Alitalo K, Ellmeier W
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 15;180(12):8048-56
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 15;180(12):8048-56
Essential roles for the Tec family kinases Tec and Btk in M-CSF receptor signaling pathways that regulate macrophage survival.
Melcher M, Unger B, Schmidt U, Rajantie IA, Alitalo K, Ellmeier W
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 15;180(12):8048-56
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 15;180(12):8048-56
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- M-CSF R/CD115 in Mouse Splenocytes. M-CSF R/CD115 was detected in immersion fixed mouse splenocytes using Mouse M-CSF R/CD115 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3818) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (orange; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.