AF1609

antibody from Novus Biologicals

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1609

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-6 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects canine IL-6 in ELISAs and Western blots. In sandwich ELISAs, less than 5% cross-reactivity with recombinant feline IL-6 and recombinant porcine IL-6 is observed, and less than 0.2% cross-reactivity with recombinant human IL-6, recombinant mouse IL-6, recombinant rat IL-6, recombinant cotton rat IL-6, and recombinant equine IL-6 is observed.

Reactivity

Canine

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of IL-6 in CLL-1390 Canine Cell Line by Flow Cytometry. CLL-1390 canine leukocytic round cell neoplasia cell line was stained with Goat Anti-Canine IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1609, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-6 and Neutralization by Canine IL-6 Antibody. Recombinant Canine IL-6 (Catalog # 1609-CL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-6 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1609). The ND₅₀ is typically 0.4-2.0 µg/mL.