Explore
Validate
Learn
ELISA
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Other assay [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AHC0469 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-6 Monoclonal Antibody (505E23C7), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is recommended as a detection antibody in Sandwich ELISA applications.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 505E23C7
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits.
Interleukin-6 is a potential therapeutic target in interleukin-6 dependent, estrogen receptor-α-positive breast cancer.
Dopamine receptors in human adipocytes: expression and functions.
Insulin stimulates interleukin-6 expression and release in LS14 human adipocytes through multiple signaling pathways.
Rodriguez E, Boelaars K, Brown K, Madunić K, van Ee T, Dijk F, Verheij J, Li RJE, Schetters STT, Meijer LL, Le Large TYS, Driehuis E, Clevers H, Bruijns SCM, O'Toole T, van Vliet SJ, Bijlsma MF, Wuhrer M, Kazemier G, Giovannetti E, Garcia-Vallejo JJ, van Kooyk Y
Communications biology 2022 Jan 11;5(1):41
Communications biology 2022 Jan 11;5(1):41
Interleukin-6 is a potential therapeutic target in interleukin-6 dependent, estrogen receptor-α-positive breast cancer.
Casneuf T, Axel AE, King P, Alvarez JD, Werbeck JL, Verhulst T, Verstraeten K, Hall BM, Sasser AK
Breast cancer (Dove Medical Press) 2016;8:13-27
Breast cancer (Dove Medical Press) 2016;8:13-27
Dopamine receptors in human adipocytes: expression and functions.
Borcherding DC, Hugo ER, Idelman G, De Silva A, Richtand NW, Loftus J, Ben-Jonathan N
PloS one 2011;6(9):e25537
PloS one 2011;6(9):e25537
Insulin stimulates interleukin-6 expression and release in LS14 human adipocytes through multiple signaling pathways.
LaPensee CR, Hugo ER, Ben-Jonathan N
Endocrinology 2008 Nov;149(11):5415-22
Endocrinology 2008 Nov;149(11):5415-22
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Effects of DA on adipokine/cytokine release. Sc explants ( panel A ), isolated mature adipocytes ( Panel B ) or differentiated primary adipocytes ( panel C ) were incubated with 1 or 100 nM DA or with 10 nM of SKF38393, a D1R/D5R agonist, for 24 hr. Leptin concentration in CM was determined by ELISA. Each value is a mean+-SEM of 5 determinations; *, p
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Time- and dose-dependent effect of insulin on IL-6 release and gene expression in undifferentiated LS14 human adipocytes. A, Cells were treated with insulin for 6 h. B, Cells were incubated with 125 nm insulin (black circles) or 10 ng/ml TNFα (white triangles) for different times. IL-6 in CM was measured by ELISA. C, Cells were incubated with 125 nm insulin, and IL-6 mRNA levels were determined by real-time PCR. Values in A and B are the mean ± sem of four determinations, and are representative of three separate experiments. IL-6 gene expression was calculated as the fold change over controls and represents three separate experiments. *, Significant differences (P < 0.05) from control.
- Conjugate
- Biotin
