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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB561 - Provider product page
- Provider
- R&D Systems
- Product name
- Cotton Rat IL-6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects cotton rat IL-6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-6, recombinant human (rh) IL-6, recombinant rat (rr) IL-6, recombinant porcine IL-6, rhCLC, rrCNTF, rmCT-1, rmIL-11, rmLIF, and rmOSM is observed.
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAL18819- Isotype
- IgG
- Antibody clone number
- 147135
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-6 and Neutralization by Cotton Rat IL-6 Antibody. Recombinant Cotton Rat IL-6 (Catalog # 561-R6) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-6 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat IL-6 Monoclonal Antibody (Catalog # MAB561). The ND50 is typically 3-15 µg/mL.
