AB-406-NA

antibody from R&D Systems

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-406-NA

Provider

R&D Systems

Product name

Mouse IL-6 Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects mouse IL-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant rat IL-6 is observed and less than 5% cross-reactivity with recombinant human IL-6, recombinant feline IL-6, recombinant bovine IL-6, and recombinant porcine IL-6 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P08505


Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Maladaptive role of IL-6 in ischemic acute renal failure.

Kielar ML, John R, Bennett M, Richardson JA, Shelton JM, Chen L, Jeyarajah DR, Zhou XJ, Zhou H, Chiquett B, Nagami GT, Lu CY
Journal of the American Society of Nephrology : JASN 2005 Nov;16(11):3315-25

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-6 and Neutralization by Mouse IL-6 Antibody. Recombinant Mouse IL-6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-6 Polyclonal Antibody (Catalog # AB-406-NA). The ND₅₀ is typically 0.05-0.15 µg/mL.