AF1575

antibody from Novus Biologicals

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AF1575

Provider

Novus Biologicals

Product name

Rabbit Polyclonal ERK1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse, and rat ERK1 in Western blots. Detects neither recombinant nor endogenous ERK2 in Western blots.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat ERK1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human/Mouse/Rat ERK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1575) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.