PA3-020

antibody from Invitrogen Antibodies

Targeting: FKBP4 FKBP52, FKBP59

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA3-020

Provider

Invitrogen Antibodies

Product name

FKBP4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA3-020 detects heat shock protein 56 (HSP56), also known as FKBP4/FKBP52, from human, monkey, mouse and rabbit tissues.

Concentration

1 mg/mL

References

Submitted references

FK506-binding protein 52 is essential to uterine reproductive physiology controlled by the progesterone receptor A isoform.

Yang Z, Wolf IM, Chen H, Periyasamy S, Chen Z, Yong W, Shi S, Zhao W, Xu J, Srivastava A, Sánchez ER, Shou W
Molecular endocrinology (Baltimore, Md.) 2006 Nov;20(11):2682-94

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Protection against ischemic brain damage in rats by immunophilin ligand GPI-1046.

Li F, Omori N, Hayashi T, Jin G, Sato K, Nagano I, Shoji M, Abe K
Journal of neuroscience research 2004 May 1;76(3):383-9

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Early decrease of the immunophilin FKBP 52 in the spinal cord of a transgenic model for amyotrophic lateral sclerosis.

Manabe Y, Warita H, Murakami T, Shiote M, Hayashi T, Omori N, Nagano I, Shoji M, Abe K
Brain research 2002 May 10;935(1-2):124-8

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Early decrease of the immunophilin FKBP 52 in the spinal cord of a transgenic model for amyotrophic lateral sclerosis.

Manabe Y, Warita H, Murakami T, Shiote M, Hayashi T, Omori N, Nagano I, Shoji M, Abe K
Brain research 2002 May 10;935(1-2):124-8

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Cholesteryl ester is transported from caveolae to internal membranes as part of a caveolin-annexin II lipid-protein complex.

Uittenbogaard A, Everson WV, Matveev SV, Smart EJ
The Journal of biological chemistry 2002 Feb 15;277(7):4925-31

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Progesterone receptor structure and function altered by geldanamycin, an hsp90-binding agent.

Smith DF, Whitesell L, Nair SC, Chen S, Prapapanich V, Rimerman RA
Molecular and cellular biology 1995 Dec;15(12):6804-12

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Progesterone receptor structure and function altered by geldanamycin, an hsp90-binding agent.

Smith DF, Whitesell L, Nair SC, Chen S, Prapapanich V, Rimerman RA
Molecular and cellular biology 1995 Dec;15(12):6804-12

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot detection of Hsp56 on human cell line S49 cytosol using Product # PA3-020.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Heat Shock Protein 56 (HSP56) was performed by loading 50 µg of the indicated whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP56 polyclonal antibody (Product # PA3-020) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Heat Shock protein 56 (HSP56, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a HSP56 polyclonal antibody (Product # PA3-020) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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