Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-30723 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIP15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG7, NIH-3T3, JC, BCL-1.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TRIP15 Polyclonal Antibody (Product # PA5-30723). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa. Lane D: HepG2. 10% SDS PAGE. TRIP15 Polyclonal Antibody (Product # PA5-30723) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TRIP15 Polyclonal Antibody (Product # PA5-30723). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. Lane C: BCL-1. 10% SDS PAGE. TRIP15 Polyclonal Antibody (Product # PA5-30723) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TRIP15 Polyclonal Antibody detects TRIP15 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRIP15 protein stained by TRIP15 Polyclonal Antibody (Product # PA5-30723) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TRIP15 Polyclonal Antibody detects TRIP15 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. TRIP15 Polyclonal Antibody (Product # PA5-30723) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TRIP15 Polyclonal Antibody detects TRIP15 protein at cytosol and nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. TRIP15 Polyclonal Antibody (Product # PA5-30723) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.