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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA504630 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504630, RRID:AB_2622490
- Product name
- SAT2 mouse monoclonal antibody, clone OTI2A3 (formerly 2A3)
- Antibody type
- Monoclonal
- Description
- SAT2 mouse monoclonal antibody, clone OTI2A3 (formerly 2A3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SAT2
- Isotype
- IgG
- Antibody clone number
- OTI2A3
- Vial size
- 100 µl
- Concentration
- 4.45 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SAT2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAT2 (RC204044, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAT2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SAT2 mouse monoclonal antibody (TA504630) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAT2(RC204044).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC204044 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SAT2 antibody(TA504630), and then analyzed by flow cytometry.
- Validation comment
- FC
