Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- AF-605-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Caspase-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Caspase-3.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Levels of caspase-3 and histidine-rich glycoprotein in the embryo secretome as biomarkers of good-quality day-2 embryos and high-quality blastocysts.
Lipid storage droplet protein 5 reduces sodium palmitate‑induced lipotoxicity in human normal liver cells by regulating lipid metabolism‑related factors.
The Effect of Long-Term Administration of Fatty Acid Amide Hydrolase Inhibitor URB597 on Oxidative Metabolism in the Heart of Rats with Primary and Secondary Hypertension.
Bok is a genuine multi-BH-domain protein that triggers apoptosis in the absence of Bax and Bak.
Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
Ellipticine derivative NSC 338258 represents a potential new antineoplastic agent for the treatment of multiple myeloma.
Photochemically mediated delivery of AdhCMV-TRAIL augments the TRAIL-induced apoptosis in colorectal cancer cell lines.
Photochemically mediated delivery of AdhCMV-TRAIL augments the TRAIL-induced apoptosis in colorectal cancer cell lines.
The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production.
Kaihola H, Yaldir FG, Bohlin T, Samir R, Hreinsson J, Åkerud H
PloS one 2019;14(12):e0226419
PloS one 2019;14(12):e0226419
Lipid storage droplet protein 5 reduces sodium palmitate‑induced lipotoxicity in human normal liver cells by regulating lipid metabolism‑related factors.
Ma X, Cheng F, Yuan K, Jiang K, Zhu T
Molecular medicine reports 2019 Aug;20(2):879-886
Molecular medicine reports 2019 Aug;20(2):879-886
The Effect of Long-Term Administration of Fatty Acid Amide Hydrolase Inhibitor URB597 on Oxidative Metabolism in the Heart of Rats with Primary and Secondary Hypertension.
Biernacki M, Łuczaj W, Jarocka-Karpowicz I, Ambrożewicz E, Toczek M, Skrzydlewska E
Molecules (Basel, Switzerland) 2018 Sep 14;23(9)
Molecules (Basel, Switzerland) 2018 Sep 14;23(9)
Bok is a genuine multi-BH-domain protein that triggers apoptosis in the absence of Bax and Bak.
Einsele-Scholz S, Malmsheimer S, Bertram K, Stehle D, Johänning J, Manz M, Daniel PT, Gillissen BF, Schulze-Osthoff K, Essmann F
Journal of cell science 2016 Jun 1;129(11):2213-23
Journal of cell science 2016 Jun 1;129(11):2213-23
Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S
Apoptosis : an international journal on programmed cell death 2010 Apr;15(4):463-73
Apoptosis : an international journal on programmed cell death 2010 Apr;15(4):463-73
Ellipticine derivative NSC 338258 represents a potential new antineoplastic agent for the treatment of multiple myeloma.
Tian E, Landowski TH, Stephens OW, Yaccoby S, Barlogie B, Shaughnessy JD Jr
Molecular cancer therapeutics 2008 Mar;7(3):500-9
Molecular cancer therapeutics 2008 Mar;7(3):500-9
Photochemically mediated delivery of AdhCMV-TRAIL augments the TRAIL-induced apoptosis in colorectal cancer cell lines.
Engesaeter BØ, Bonsted A, Lillehammer T, Engebraaten O, Berg K, Maelandsmo GM
Cancer biology & therapy 2006 Nov;5(11):1511-20
Cancer biology & therapy 2006 Nov;5(11):1511-20
Photochemically mediated delivery of AdhCMV-TRAIL augments the TRAIL-induced apoptosis in colorectal cancer cell lines.
Engesaeter BØ, Bonsted A, Lillehammer T, Engebraaten O, Berg K, Maelandsmo GM
Cancer biology & therapy 2006 Nov;5(11):1511-20
Cancer biology & therapy 2006 Nov;5(11):1511-20
The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production.
Chae JJ, Wood G, Masters SL, Richard K, Park G, Smith BJ, Kastner DL
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 27;103(26):9982-7
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 27;103(26):9982-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Caspase-3 by Simple WesternTM. Detection of Human Caspase-3 by Simple WesternTM.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Caspase-3 by Western Blot. Detection of Human and Mouse Caspase-3 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation of Human Caspase-3. Jurkat human acute T cell leukemia cell line was treated with indicated concentrations of Staurosporine for 0 or 4 hours. Caspase-3 was immunoprecipitated from lysates of 1-2 x 106 cells following incubation with 1 or 4 µg Goat Anti-Human Caspase-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-605-NA) overnight at 4 ºC. Caspase-3-antibody complexes were absorbed using Protein G expressing Staph cells (Sigma). Immunoprecipitated Caspase-3 was detected by Western blot using 0.5 µg/mL Goat Anti-Human Caspase-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-605-NA). View our recommended buffer recipes for immunoprecipitation.