NBP2-36765

antibody from Novus Biologicals

Targeting: PDIA3 ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-36765

Provider

Novus Biologicals

Product name

Mouse Monoclonal ERp57/PDIA3 Antibody

Antibody type

Monoclonal

Description

Protein A purified. Specificity of human ERp57/PDIA3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Mouse

Antigen sequence

KKFISDKDASIVGFF


Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Vitamin D receptor is present on the neuronal plasma membrane and is co-localized with amyloid precursor protein, ADAM10 or Nicastrin.

Dursun E, Gezen-Ak D
PloS one 2017;12(11):e0188605

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDIA3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human thyroid gland shows moderate to strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in human thyroid gland and skeletal muscle tissues. Corresponding PDIA3 RNA-seq data are presented for the same tissues.